1:00pm |
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High-Throughput Self-Interaction Chromatography: Applications Protein Solubility & Stability
(VTC)
Larry DeLucas, OD, PhD
Professor and Director
Center for Biophysical Sciences and Engineering
University of Alabama, Birmingham
Birmingham, Alabama
A seminar in the Research Institute Seminar Series
The physical stability of a protein generally depends on suppression of aggregation. In either case, it is ultimately the protein-protein interactions in the specified solvent condition that dictates the process outcome. A novel technology, High-throughput Self-interaction Chromatography (HSC) is presented for measuring protein-protein interactions. The combination of the HSC technology and a balanced screen of different additives in solution are used to measure protein-protein interactions. The results are input into an artificial neural network program that predicts new solution conditions with novel additive combinations and concentrations. Examples demonstrating its utility for rapidly optimizing protein solubility and physical stability will be presented.
In addition, Dr. DeLucas will briefly describe a new concept that involves use of embryonic stem cells to express milligram quantities of membrane proteins in an essentially purified form. The presentation will conclude with a summary of a major protein crystallization initiative currently underway on the International Space Station.
Live Webcast: http://research.vtc.vt.edu/live-webcast
Hosted by: Michael J. Friedlander, PhD, Executive Director, Virginia Tech Carilion Research Institute
More information...
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540-526-2013